PCR Machine

PCR Machine – Primer Design

Easy extraction from virtually any sample type

The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to RNA/DNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR. Its fast, and incredibly easy to perform.

Hubungi Kami

Suitable sample types

  • Whole blood
  • Serum
  • Plasma
  • Saliva
  • Sputum
  • Faeces
  • Urine
  • Tissue
  • Food, eg meat, fish or milk.
  • Bacterial culture broth

PCR Machine – Kyratec

The SuperCycler Trinity is a high performance thermal cycling system configured and optimised for industry standard 200ul individual or strip tube (domed or flat-capped) or 96-well plates (low or high skirt) with strip caps or adhesive film seals. It incorporates state of the art electronics, precision quality peltier devices and a flexible user interface.

Platform

  • Trinity Series Powerful Thermal Engine
  • The TripleZone Advantage
  • Heated Lid Evaporation Control
  • Touch Screen Graphical User Interface
  • Compact Footprint
  • USB Connectivity

Interface

The SuperCycler software implements a powerful thermal profile engine. A profile may contain up to 100 events. Each ‘event’ can be either a hold at temperature, pause, ramp or 2 to 5 step cycling with up to 100 repeats. Any event or step can contain gradient*, touchdown or long range features. An almost unlimited number of profiles may be stored on the device for re-running. Despite its high level of capabilities profile setup is straightforward.

  • Live Graphing
  • Manual Control
  • Auto Restart
  • Quickstart Wizard
  • USB Connectivity
  • User Accounts
  • Pause
  • Long Range
  • Touch Down/Up
  • On Screen Help
  • Run Reporting

Specifications

  • Thermal Cycling Technology: High performance active heating and cooling using 6 quality peltier elements arranged as 3 independant control zones
  • Temperature Range: 4°C – 99°C
  • Temperature Accuracy: ±0.5°C over full range, typically < ±0.1°C at 60°C
  • Temperature Uniformity: ±0.3°C, 30 seconds after target (range 40°C – 90°C) Typically < ±0.1°C at 60°C
  • Temperature Resolution: 0.1°C increments
  • Heating/Cooling Rate: 5°C/sec typical heat & cool between denat & aneal (block). 7°C/sec Peak (block).
  • Well Configuration: 96-well block supporting: 0.2 mL tubes or strip tubes with flat or domed caps; 96-well high-or low skirt plates with strip caps, adhesive cover, or oil overlay
  • Recommended Reaction Volume: 5 – 60ul
  • Thermal Gradient* (SC300G): Programmable 0–24°C across block width (12 wells)
  • Condensation Control: Automatic utilising applied pressure heated lid
  • Heated Lid Temperature Range: Controllable 60°C – 115°C
  • Dimensions: Width: 180mm (7”); Depth: 285mm (11.2”); 350mm (13.8”) including cables; Height: 190mm (7.5”) lid closed; 340mm (13.4”) lid open
  • Weight: 5.5kg (11 lbs)
  • Colour: Pewter on black
  • Electrical: 100–240 VAC @ 4 Amp (50/60 Hz) Automatic voltage sense, standard IEC Inlet plug
  • External Connectivity Interface: USB interface to Windows based PC; USB host port – file transfer to and from USB memory stick – mouse/keyboard connection – printer (potential future software release)
  • Internal Interface: Embedded graphical controller with 7” widescreen touch sensitive colour backlit display
  • Software: Supplied with unlimited user licenses, Free upgrades available via web download
  • Internal memory: 256MB, enough for 10,000+ saved profiles
  • Functionality: Multiple thermal zones, Touch Down/Up, Long Range, Thermal Gradient*, Program Pauses, Temperature Graphing, On-screen Help, User Accounts, Profile Load and Saving, Manual Mode, USB File Transfer, Post run reporting, Auto restart and more.
  • Included Accessories: Power Cable, User Manual, Touch Screen Stylus
  • Ordering Information: Triple-Zone SC300T; Gradient* (Not available in all regions) SC300G; Uni-Block SC300U